Journal: Frontiers in Pharmacology

Article Title: Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway

doi: 10.3389/fphar.2025.1567762

Figure Lengend Snippet: TQ Ameliorates Oxidative Stress and Apoptosis in Rat Perforator Flaps After Ischemia-Reperfusion Injury. (A) Immunohistochemical staining images of SOD1 in the control and TQ groups (scale bar, 50 μm). (B) Quantitative analysis of SOD1 absorbance in the control and TQ groups. (C) Western blotting to detect the expression of oxidative stress-related proteins: eNOS, HO1, and SOD1 in flap tissues of the control and TQ groups. (D) Quantitative analysis of oxidative stress-related protein blots. (E) Immunohistochemical staining images of C-CASP3 in the control and TQ groups (scale bar, 50 μm). (F) Quantitative analysis of C-CASP3 absorbance in the control and TQ groups. (G) Western blotting to detect the expression of apoptosis-related proteins: Bax, C-CASP3, and Bcl-2 in flap tissues of the control and TQ groups. (H) Quantitative analysis of apoptosis-related protein blots. Significance: *p < 0.05 and **p < 0.01. Data are presented as mean ± SEM, with five rats per group.

Article Snippet: Anti-cadherin 5 is provided by Wuhan Boster Biological Technology, Ltd. Primary antibodies against GAPDH, HO1, SOD1, Bax, Bcl-2, GSDMD-N, and VEGF are sourced from The Proteintech Group (Chicago, USA).

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway

doi: 10.3389/fphar.2025.1567762

Figure Lengend Snippet: EX527 Reverses the Inhibitory Effects of TQ on Pyroptosis, Oxidative Stress, and Apoptosis in Rat Perforator Flaps After Ischemia-Reperfusion Injury. (A) Western blotting to detect the expression of pyroptosis-related proteins: NLRP3, GSDMD-N, Caspase-1, IL-1β, IL-18, and ASC in flap tissues of the control, TQ, and TQ+EX527 groups. (B) Quantitative analysis of pyroptosis-related protein blots. (C) GSDMD-N protein expression levels in flap tissues of rats in the control, TQ, and TQ+EX527 groups measured by immunofluorescence (scale bar: 20 µm). (D) Fluorescence intensity of GSDMD-N expression in each group analyzed using Image J. (E) Western blotting to detect the expression of oxidative stress-related proteins: eNOS, HO1, and SOD1 in flap tissues of the control, TQ, and TQ+EX527 groups. (F) Quantitative analysis of oxidative stress-related protein blots. (G) Western blotting to detect the expression of apoptosis-related proteins: Bax, C-CASP3, and Bcl-2 in flap tissues of the control, TQ, and TQ+EX527 groups. (H) Quantitative analysis of apoptosis-related protein blots. Significance: *p < 0.05 and **p < 0.01. Data are presented as mean ± SEM, with five rats per group.

Article Snippet: Anti-cadherin 5 is provided by Wuhan Boster Biological Technology, Ltd. Primary antibodies against GAPDH, HO1, SOD1, Bax, Bcl-2, GSDMD-N, and VEGF are sourced from The Proteintech Group (Chicago, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Fluorescence

Journal: International Journal of Medical Sciences

Article Title: Visnagin Protects Against Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting Oxidative Stress and Reducing Ferroptosis

doi: 10.7150/ijms.125642

Figure Lengend Snippet: Visnagin suppresses Akt and Nrf2 phosphorylation and downregulates antioxidative enzyme expression in renal tissues of LPS-induced AKI mice. Representative western blot images and quantitative analysis of phosphorylated Akt, phosphorylated Nrf2, and antioxidant-related proteins (HO-1, SOD, catalase, and GPX4) in renal tissues. Protein expression levels were normalized to the corresponding loading controls. Data are presented as mean ± SD from biological replicates derived from independent experiments (n = 3). Statistical significance is indicated as * P <.05 versus control; # P <.05 versus LPS.

Article Snippet: The proteins were subsequently transferred onto polyvinylidene difluoride membranes, which were blocked with 5% nonfat milk for 1 h. The membranes were then incubated with primary antibodies ACSL4 (Cat: SC-365230, Santa Cruz, CA, USA), TfR1 (Cat: SC-393719, Santa Cruz, CA, USA), p-Akt (Cat: SC-514032, Santa Cruz, CA, USA), Akt (Cat: SC-81434, Santa Cruz, CA, USA), p-Nrf2 (Cat: PA5-67520, Invitrogen, Thermo Fisher Scientific, MA, USA), Nrf2 (Cat: SC-365949, Santa Cruz, CA, USA), HO-1 (Cat: SC-390991, Santa Cruz, CA, USA), SOD (Cat: 10269-1-AP, Proteintech, IL, USA), Catalase (Cat: 21260-1-AP, Proteintech, IL, USA), GPX4 (Cat: SC-166570, Santa Cruz, CA, USA) and β-actin (Cat: SC-47778, Santa Cruz, CA, USA) overnight at 4°C.

Techniques: Phospho-proteomics, Expressing, Western Blot, Derivative Assay, Control